Ku70 + Ku80 antibody

Catalog No. ABIN3026515

The Mouse Monoclonal anti-Ku70 + Ku80 antibody (Clone KU729) (ABIN3026515) specifically detects Ku70 + Ku80 in IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for Ku70 + Ku80 antibody (ABIN3026515)

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ku70 + Ku80 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: KU729

Product Details anti-Ku70 + Ku80 Antibody

Purpose: Ku70 + Ku80 Antibody (azide and preservative free)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken

Characteristics: This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Purification: Protein G purified monoclonal antibody

Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Ku70 + Ku80

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Alternative Name: Ku70 + Ku80

Background: This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.