Ku70 + Ku80 antibody

Catalog No. ABIN6251789

The Mouse Monoclonal anti-Ku70 + Ku80 antibody (Clone KU729) (ABIN6251789) specifically detects Ku70 + Ku80 in IF, FACS and ICC. The antibody is reactive with Human and Primate samples.

Quick Overview for Ku70 + Ku80 antibody (ABIN6251789)

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ku70 + Ku80 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: KU729

Product Details anti-Ku70 + Ku80 Antibody

Specificity: Does not react with mouse, rat, chicken

Purification: Protein G purified monoclonal antibody

Immunogen: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: 0.5-1 μg/10e6 cells

IF: 0.5-1.0 μg/mL

Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Ku70 + Ku80

Target: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Alternative Name: Ku70 + Ku80

Background: This antibody recognizes a dimer of two proteins of 70  kDa (Ku70) and ~80  kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460  kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Gene ID: 2547

UniProt: Q6FG89