MUC1 antibody

Catalog No. ABIN3026736

The Mouse Monoclonal anti-MUC1 antibody is suitable to detect MUC1 in samples from Human. It has been validated for FACS, IF and IHC (p).

Quick Overview for MUC1 antibody (ABIN3026736)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MUC1-845

Product Details anti-MUC1 Antibody

Characteristics: This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Purification: Protein G affinity chromatography

Purity: Protein G affinity chromatography

Immunogen: Human milk fat globule membranes were used as the immunogen for this EMA antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.

1. No pretreatment needed for FFPE staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the EMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: EMA

Background: This antibody recognizes proteins in MW range of 265-400  kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Gene ID: 4582

Pathways: Negative Regulation of intrinsic apoptotic Signaling