ICAM-3/CD50 antibody
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- Target See all ICAM-3/CD50 (ICAM3) Antibodies
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ICAM-3/CD50 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR)
- Characteristics
- This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
- Purification
- Protein G affinity chromatography
- Immunogen
- Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
- Clone
- 186-2G9
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product ICAM3 Primary Antibody
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- Application Notes
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD50 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Functional Activity (Blocking): Blocks CD50-CD11a interaction,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Alternative Name
- CD50 (ICAM3 Products)
- Synonyms
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- Background
- This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI). It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
- Gene ID
- 3385
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