HIF1A antibody (AA 703-732)

Catalog No. ABIN3028724

The Rabbit Polyclonal anti-HIF1A antibody has been validated for WB and IHC (p). It is suitable to detect HIF1A in samples from Human, Mouse and Rat.

Quick Overview for HIF1A antibody (AA 703-732) (ABIN3028724)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HIF1A Antibody

Binding Specificity: AA 703-732

Purification: Antigen affinity

Immunogen: Amino acids 703-732 (EEELNPKILALQNAQRKRKMEHDGSLFQAV-human) were used as the immunogen for this HIF-1-alpha antibody.

Isotype: IgG

Application Details

Application Notes: Titration of the HIF-1-alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: The lyophilized HIF-1-alpha antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF-1-alpha

Background: Hypoxia-inducible factor 1a (HIF-1-alpha) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. It is crucial for the cellular response to hypoxia and plays a pivotal role in cellular adaptation to changes in oxygen availability.

Gene ID: 3091

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect