RELB antibody (AA 393-413)

Catalog No. ABIN3029550

Product Details anti-RELB Antibody

Target: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Binding Specificity: AA 393-413

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RELB antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids 393-413 (LPFTYLPRDHDSYGVDKKRKR-human) were used as the immunogen for this RelB antibody (100% mouse homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the RelB antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Rel B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for RELB

Target: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Alternative Name: RelB (RELB Products)

Synonyms: shep antibody, I-REL antibody, IREL antibody, REL-B antibody, XRelB antibody, relb-A antibody, GC-rich antibody, avian reticuloendotheliosis viral (v-rel) oncogene related B antibody, RELB proto-oncogene, NF-kB subunit antibody, v-rel avian reticuloendotheliosis viral oncogene homolog B S homeolog antibody, Relb antibody, RELB antibody, relb.S antibody

Background: RelB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. By RT-PCR and immunocytochemical analyses, Clark et al.(1999) showed that protein expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al.(2008) found that overexpression of RelB, but not RelA, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classic pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using RelB -/- mice, they showed that the protein itself was required for RankL-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both RelB -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al.(2008) concluded that the alternative NF-kappa-B pathway, via RelB, plays an essential and unique role in RankL signaling toward osteoclast development.

UniProt: Q01201

Pathways: NF-kappaB Signaling, RTK Signaling