PIK3R1 antibody (AA 447-461)

Catalog No. ABIN3029749

This Rabbit Polyclonal antibody specifically detects PIK3R1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIK3R1 antibody (AA 447-461) (ABIN3029749)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIK3R1 Antibody

Binding Specificity: AA 447-461

Purpose: PI3K Antibody (p85 beta)

Purification: Antigen affinity

Immunogen: Amino acids 447-461 (KVYHQQYQDKSREYD-beta specific) was used as the immunogen for this PI3K antibody (100% human, mouse and rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PI3K antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PI3K antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PI3K

Background: Phosphatidylinositol 3-kinase regulatory subunit 2, also called p85-Beta, PIK3R2 and PIK3, is an enzyme that in humans is encoded by the PIK3R2 gene. The NS1 protein from various influenza strains bind p85-beta, but not p85-alpha. Binding of p85-beta and activation of PI3K required tyr89 of NS1, and mutant viruses expressing NS1 with a tyr89-to-phe substitution grew more slowly in cell culture than wildtype viruses. Using mouse embryonic fibroblasts, Park et al. showed that, in addition to regulating PI3K function, p85-alpha/beta regulated the function of Xbp1s, a transcription factor that orchestrates the unfolded protein response (UPR) following endoplasmic reticulum (ER) stress. Overexpression of the protein in livers of ob/ob mice increased glucose tolerance and reduced blood glucose concentrations.

UniProt: O00459

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect