Aquaporin 4 antibody (AA 258-274)

Catalog No. ABIN3030007

This Rabbit Polyclonal antibody specifically detects Aquaporin 4 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Aquaporin 4 antibody (AA 258-274) (ABIN3030007)

Target: Aquaporin 4 (AQP4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aquaporin 4 Antibody

Binding Specificity: AA 258-274

Purification: Antigen affinity

Immunogen: Amino acids 258-274 (FKRRFKEAFSKAAQQTK) were used as the immunogen for this Aquaporin 4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Aquaporin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Aquaporin 4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aquaporin 4

Background: Aquaporin 4, also known as Mercurial-Insensitive Water Channel (MIWC), is protein which in humans is encoded by the AQP4 gene. The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. By fluorescence in situ hybridization, Lu et al.(1996) determined that the AQP4 gene maps to 18q11.2-q12.1. By interspecific backcross analysis, Turtzo et al.(1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18. Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al.(1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers. Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.

UniProt: P55087

Pathways: Sensory Perception of Sound