CTBP1 antibody (AA 425-440)

Catalog No. ABIN3030237

Product Details anti-CTBP1 Antibody

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Binding Specificity: AA 425-440

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Amino acids 425-440 (QTVKPEADRDHASDQL-human) were used as the immunogen for this CtBP1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CtBP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CtBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CTBP1

Target: CTBP1 (C-terminal Binding Protein 1 (CTBP1))

Alternative Name: CtBP1 (CTBP1 Products)

Synonyms: BARS antibody, CtBP1-L antibody, CtBP1-S antibody, CtBP3/BARS antibody, D4S115h antibody, D5H4S115 antibody, D5H4S115E antibody, 50-kDaBFA-inducedADP-ribosylatedsubstrate antibody, BARS-50 antibody, Bars antibody, wu:fi43a11 antibody, CtBP antibody, ctbp1-A antibody, xctbp1 antibody, bars antibody, CTBP1 antibody, C-terminal binding protein 1 antibody, C-terminal binding protein 1 L homeolog antibody, CTBP1 antibody, Ctbp1 antibody, ctbp1 antibody, ctbp1.L antibody

Background: C-terminal-binding protein 1 is encoded by the CTBP1 gene. It binds to the C-terminus of adenovirus E1A proteins. It is a transcriptional repressor(corepressor) and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and -2 associate with the PLDLSL domain of delta EF1, a cellular zinc finger-homeodomain protein, and thereby enhances delta EF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

UniProt: Q13363

Pathways: Retinoic Acid Receptor Signaling Pathway