CDK2 antibody (AA 277-298)

Catalog No. ABIN3030607

This Rabbit Polyclonal antibody specifically detects CDK2 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDK2 antibody (AA 277-298) (ABIN3030607)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK2 Antibody

Binding Specificity: AA 277-298

Purification: Antigen affinity

Immunogen: Amino acids 277-298 (AKAALAHPFFQDVTKPVPHLRL) were used as the immunogen for this Cdk2 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cdk2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Cdk2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: Cdk2

Background: Cyclin-Dependent Kinase 2 is also know as p33. The protein is highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that< a href=../search_result.php?search_txt=cdk2>Cdk2is the human homolog of Eg1. The gene is mapped to 12q13, the same region to which the< a href=../search_result.php?search_txt=cdk4>Cdk4gene maps. Human Cyclin A binds independently to two kinases, p34/Cdc2) or p33/Cdk2. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/Cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65 % sequence identity with p34(cdc2). Cdk2 plays a unique role in cell cycle regulation of vertebrate cells.

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA