CDK4 antibody (C-Term)

Catalog No. ABIN3030611

The Rabbit Polyclonal anti-CDK4 antibody has been validated for WB. It is suitable to detect CDK4 in samples from Human, Mouse and Rat.

Quick Overview for CDK4 antibody (C-Term) (ABIN3030611)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK4 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of mouse Cdk4 (RISAFRALQHSYLHKEESDAE) was used as the immunogen for this Cdk4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Cdk4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4

Background: Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.

UniProt: P30285

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size