CISH antibody (AA 241-258)

Catalog No. ABIN3030694

This Rabbit Polyclonal antibody specifically detects CISH in WB, ICC and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CISH antibody (AA 241-258) (ABIN3030694)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CISH Antibody

Binding Specificity: AA 241-258

Purification: Antigen affinity

Immunogen: Amino acids 241-258 (LPLPRRMADYLRQYPFQL) were used as the immunogen for this CISH antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CISH antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CISH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: CISH

Background: Cytokine inducible SH2-containing protein, also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling (SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CISH expression was upregulated by lipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CISH. Gain- and loss-of-function studies showed that CISH accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

UniProt: Q9NSE2

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus