BMI1 antibody

Catalog No. ABIN303071

The Goat Polyclonal anti-BMI1 antibody is suitable to detect BMI1 in samples from Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Cat and Frog. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for BMI1 antibody (ABIN303071)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Monkey, Cat, Frog

Host: Goat

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-BMI1 Antibody

Specificity: This antibody reacts to DNA-binding protein Bmi-1.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 2,5 μg/mL. Western Blot: 1: 500 - 1: 3000. ELISA: 1: 5000 - 30000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1 / RNF51

Background: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gene ID: 100532731

NCBI Accession: NP_001190991

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy