DDB1 antibody (AA 198-213)

Catalog No. ABIN303090

The Rabbit Polyclonal anti-DDB1 antibody (ABIN303090) specifically detects DDB1 in WB, IP, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for DDB1 antibody (AA 198-213) (ABIN303090)

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DDB1 Antibody

Binding Specificity: AA 198-213

Specificity: This antibody reacts to amino acids 198-213 of DDB1 (internal) coupled to KLH.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Delipidation and Defibrination.

Immunogen: Synthetic peptide (coupled to KLH) corresponding to amino acids 198-213 of Human DDB1 (internal).

Application Details

Application Notes: ELISA: 1/2000-1/10000. Immunoprecipitation. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/500. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1 / XAP1

Background: DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV-damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein

Gene ID: 100290337

NCBI Accession: NP_001914

UniProt: Q16531

Pathways: DNA Damage Repair