PNKP antibody (Internal Region)

Catalog No. ABIN303129

The Rabbit Polyclonal anti-PNKP antibody (ABIN303129) specifically detects PNKP in WB, IF, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for PNKP antibody (Internal Region) (ABIN303129)

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PNKP Antibody

Binding Specificity: Internal Region

Specificity: ,Species:Human, Dog, Mouse and Rat.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to an internal region of human PNK protein.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunofluorescence. Western Blot (1/300-1/1500). ELISA (1/3000-1/12000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP

Background: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5 phosphorylation of nucleic acids and can have associated 3 phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase

Gene ID: 11284

NCBI Accession: NP_009185

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation