IL-6 antibody (AA 195-211)

Catalog No. ABIN3031290

This anti-IL-6 antibody is a Rabbit Polyclonal antibody detecting IL-6 in WB. Suitable for Mouse and Rat.

Quick Overview for IL-6 antibody (AA 195-211) (ABIN3031290)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-6 Antibody

Binding Specificity: AA 195-211

Purification: Antigen affinity

Immunogen: Amino acids 195-211 (KALEEFLKVTMRSTRQT) were used as the immunogen for this IL-6 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the IL-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the IL-6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL6 gene to chromosome 7p15.

UniProt: P20607

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome