POLK antibody (AA 817-830)

Catalog No. ABIN303130

This Goat Polyclonal antibody specifically detects POLK in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLK antibody (AA 817-830) (ABIN303130)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-POLK Antibody

Binding Specificity: AA 817-830

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunofluorescence. Western Blot (1/500-1/3000). ELISA (1/2000-1/10000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.8 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: DNA Polymerase kappa

Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.Synonyms: DINB protein, DINB1, DINP, POLK

Gene ID: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: DNA Damage Repair