CD11b antibody (N-Term)

Catalog No. ABIN3031366

This Rabbit Polyclonal antibody specifically detects CD11b in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD11b antibody (N-Term) (ABIN3031366)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD11b Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human CD11b (MEQLKKSKTLFSLMQY) was used as the immunogen for this CD11b antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD11b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: ITGAM (Integrin Alpha-M), also called CD11b, MAC-1 or Mo1 alpha subunit (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2(alphaMbeta2) molecule. A major surface antigen family on human leukocytes includes complement receptor type 3(CR3A, also called ITGAM, Mac1, or Mo1), lymphocyte function-associated(LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX). By in situ hybridization, Corbi et al.(1988) demonstrated that the genes encoding the alpha subunits of LFA1(ITGAL), Mac1, and p150, 95(ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11. Callen et al.(1991) narrowed the assignment to 16p11.2. Inflammation plays an essential role in the initiation and progression of atherosclerosis. Simon et al.(2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation