MAP2K4 antibody (C-Term)

Catalog No. ABIN3031843

This Rabbit Polyclonal antibody specifically detects MAP2K4 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP2K4 antibody (C-Term) (ABIN3031843)

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP2K4 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human MKK4/MEK4 (ELLKHPFILMYEERAVEVA) was used as the immunogen for this MKK4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the MKK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the MKK4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for MAP2K4

Target: MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4))

Alternative Name: MKK4 MEK4

Background: Dual specificity mitogen-activated protein kinase kinase 4 (MAP2K4), also called MEK4, SEK1, JNKK1 and MKK4, is an enzyme that in humans is encoded by the MAP2K4 gene. It is mapped to 17p12. This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK1. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. MAP2K4 was a specific activator of JNK1, JNK2, and p38, and it has been shown to interact with FLNC, MAPK8, MAPK8IP3 and AKT1.

UniProt: P45985

Pathways: MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling