PPARA antibody (AA 1-18)

Catalog No. ABIN303185

The Rabbit Polyclonal anti-PPARA antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect PPARA in samples from Human, Mouse and Monkey.

Quick Overview for PPARA antibody (AA 1-18) (ABIN303185)

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PPARA Antibody

Binding Specificity: AA 1-18

Specificity: ,Species:Chimpanzee, Human, Monkey and Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to Amino Acids 1-18 of mouse PPAR alpha.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (20 μg/mL). Western Blot (1/500-1/2000). ELISA (1/8000-1/32000).

Restrictions: For Research Use only

Handling

Concentration: 1.7 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.

Target Details for PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Alternative Name: PPAR-alpha

Background: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C1, Nuclear receptor subfamily 1 group C member 1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha

Gene ID: 19013

NCBI Accession: NP_001106889

UniProt: P23204

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C