PMVK antibody (Middle Region)

Catalog No. ABIN3032231

The Rabbit Polyclonal anti-PMVK antibody is suitable to detect PMVK in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for PMVK antibody (Middle Region) (ABIN3032231)

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PMVK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PMVK Antibody

Binding Specificity: Middle Region

Purpose: PMVK Antibody

Sequence: EQSRQQRGWV FTPGVDD

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human PMVK (EQSRQQRGWVFTPGVDD) was used as the immunogen for this PMVK antibody (100% homologous in human and mouse).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PMVK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PMVK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PMVK

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Alternative Name: PMVK

Background: Phosphomevalonate kinase is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence(PTS-1), Ser-Arg-Leu, at the C terminus of the protein.

UniProt: Q15126