PF4 antibody (AA 85-101)

Catalog No. ABIN3032246

The Rabbit Polyclonal anti-PF4 antibody has been validated for WB and IHC (p). It is suitable to detect PF4 in samples from Human.

Quick Overview for PF4 antibody (AA 85-101) (ABIN3032246)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PF4 Antibody

Binding Specificity: AA 85-101

Purification: Antigen affinity

Immunogen: Amino acids 85-101 (DLQAPLYKKIIKKLLES-human) were used as the immunogen for this CXCL4 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the CXCL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CXCL4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: CXCL4

Background: Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. It belongs to the CXC chemokine family. The gene contains 3 exons spanning approximately 1 kb. It is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the gene is mapped to chromosome 4q12-q21. CXCL4s major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, the cytokine probably has a role in inflammation and wound repair.

UniProt: P02776