PIAS1 antibody (AA 636-651)

Catalog No. ABIN3032369

The Rabbit Polyclonal anti-PIAS1 antibody has been validated for WB, ICC and IHC (p). It is suitable to detect PIAS1 in samples from Human, Mouse and Rat.

Quick Overview for PIAS1 antibody (AA 636-651) (ABIN3032369)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIAS1 Antibody

Binding Specificity: AA 636-651

Purification: Antigen affinity

Immunogen: Amino acids 636-651 (DTASIFGIIPDIISLD-human) were used as the immunogen for this PIAS1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PIAS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PIAS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. It contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PIAS1 can also function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

UniProt: O75925

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C