RAB8A antibody (C-Term)

Catalog No. ABIN3032426

The Rabbit Polyclonal anti-RAB8A antibody has been validated for WB and IHC (p). It is suitable to detect RAB8A in samples from Human, Mouse and Rat.

Quick Overview for RAB8A antibody (C-Term) (ABIN3032426)

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB8A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAB8A Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Rab8A (KAKMDKKLEGNSPQ) was used as the immunogen for this Rab8 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Rab8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Rab8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for RAB8A

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Alternative Name: Rab8

Background: Ras-related protein Rab8 is a protein that in humans is encoded by the RAB8A gene. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. Rab8 regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of Rab8 into the cilium during assembly of this organelle. It has been shown to interact with Optineurin and MAP4K2.

UniProt: P61006

Pathways: M Phase, Regulation of long-term Neuronal Synaptic Plasticity, SARS-CoV-2 Protein Interactome