CD11b antibody (PE)

Catalog No. ABIN303522

This anti-CD11b antibody is a Mouse Monoclonal antibody detecting CD11b in FACS. Suitable for Human.

Quick Overview for CD11b antibody (PE) (ABIN303522)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MEM-174

Product Details anti-CD11b Antibody (PE)

Specificity: The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Purification: Size-exclusion chromatography

Immunogen: Human granulocytes

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation