CDC25C antibody (AA 175-473)

Catalog No. ABIN3042352

This Rabbit Polyclonal antibody specifically detects CDC25C in WB. It exhibits reactivity toward Human and Rat and has been mentioned in 2+ publications.

Quick Overview for CDC25C antibody (AA 175-473) (ABIN3042352)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25C Antibody

Binding Specificity: AA 175-473

Purpose: Anti-Cdc25C Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cdc25C Antibody Picoband® (ABIN3042352). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdc25C recombinant protein (Position: K175-P473). Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe)". 2. Gould KL, Moreno S, Tonks NK, Nurse P (Feb 1991). "Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase". Science 250 (4987): 1573-6. 3. Sartor H, Ehlert F, Grzeschik KH, et al. (1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively". Genomics 13(3): 911-2.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CDC25C antibody (ABIN3042352)

Lin, Zhu, Sun, Yang, Sun, Li, Cui, Zheng, Yu: "Prostate cancer cell proliferation is suppressed by microRNA-3160-5p via targeting of F-box and WD repeat domain containing 8." in: Oncology letters, Vol. 15, Issue 6, pp. 9436-9442, (2018) (PubMed).

Ling, Lu, He, Xiao, Jiang, Su: "Diallyl disulfide selectively causes checkpoint kinase-1 mediated G2/M arrest in human MGC803 gastric cancer cell line." in: Oncology reports, Vol. 32, Issue 5, pp. 2274-82, (2014) (PubMed).

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background:

Synonyms: M-phase inducer phosphatase 3,3.1.3.48,Dual specificity phosphatase Cdc25C,CDC25C,

Tissue Specificity: Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .

Background: M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It s dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Molecular Weight: 60 kDa

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase