Menin antibody (AA 301-615)

Catalog No. ABIN3042359

The Rabbit Polyclonal anti-Menin antibody has been validated for WB. It is suitable to detect Menin in samples from Human and Mouse.

Quick Overview for Menin antibody (AA 301-615) (ABIN3042359)

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Menin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Menin Antibody

Binding Specificity: AA 301-615

Purpose: Anti-Menin/MEN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Menin/MEN1 Antibody Picoband® (ABIN3042359). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Menin recombinant protein (Position: P301-L615). Human Menin shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Menin, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Canaff, L., Vanbellinghen, J.-F., Kaji, H., Goltzman, D., Hendy, G. N. Impaired transforming growth factor-beta (TGF-beta) transcriptional activity and cell proliferation control of a menin in-frame deletion mutant associated with multiple endocrine neoplasia type 1 (MEN1). J. Biol. Chem. 287: 8584-8597, 2012. 2. Kaji, H., Canaff, L., Lebrun, J.-J., Goltzman, D., Hendy, G. N. Inactivation of menin, a Smad3-interacting protein, blocks transforming growth factor type beta signaling. Proc. Nat. Acad. Sci. 98: 3837-3842, 2001. 3. Zablewska, B., Bylund, L., Mandic, S. A., Fromaget, M., Gaudray, P., Weber, G. Transcription regulation of the multiple endocrine neoplasia type 1 gene in human and mouse. J. Clin. Endocr. Metab. 88: 3845-3851, 2003.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Menin

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Alternative Name: MEN1

Background:

Synonyms: Menin,MEN1,SCG2,

Tissue Specificity: Ubiquitous.

Background: The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. What's more, MEN1 was essential to maintain MLL-associated myeloid transformation.

Molecular Weight: 68 kDa

Gene ID: 4221

UniProt: O00255

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity