EPH Receptor B1 antibody (N-Term)

Catalog No. ABIN3042375

The Rabbit Polyclonal anti-EPH Receptor B1 antibody (ABIN3042375) specifically detects EPH Receptor B1 in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EPH Receptor B1 antibody (N-Term) (ABIN3042375)

Target: EPH Receptor B1 (EPHB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-EPH Receptor B1 Antibody

Binding Specificity: AA 56-88, N-Term

Purpose: Anti-Eph receptor B1/EPHB1 Antibody

Sequence: RTYQVCNVFE PNQNNWLLTT FINRRGAHRI YTE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Eph receptor B1/EPHB1 Antibody (ABIN3042375). Tested in Flow Cytometry, IHC WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Eph receptor B1 , identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EPH Receptor B1

Target: EPH Receptor B1 (EPHB1)

Alternative Name: EPHB1

Background:

Synonyms: Ephrin type-B receptor 1,2.7.10.1,ELK,EPH tyrosine kinase 2,EPH-like kinase 6,EK6,hEK6,Neuronally-expressed EPH-related tyrosine kinase,NET,Tyrosine-protein kinase receptor EPH-2,EPHB1,ELK, EPHT2, HEK6, NET,

Tissue Specificity: Preferentially expressed in brain.

Background: Ephrin type-B receptor 1 is a protein that in humans is encoded by the EPHB1 gene. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Molecular Weight: 120 kDa

Gene ID: 2047

UniProt: P54762

Pathways: RTK Signaling