NCOA1 antibody (AA 614-826)

Catalog No. ABIN3042385

This Rabbit Polyclonal antibody specifically detects NCOA1 in WB, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for NCOA1 antibody (AA 614-826) (ABIN3042385)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NCOA1 Antibody

Binding Specificity: AA 614-826

Purpose: Anti-KAT13A/SRC1/NCOA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-KAT13A/SRC1/NCOA1 Antibody (ABIN3042385). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KAT13A recombinant protein (Position: H614-Q826). Human KAT13A shares 92% amino acid (aa) sequence identity with mouse KAT13A.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Onate, S. A., Tsai, S. Y., Tsai, M.-J., O'Malley, B. W. Sequence and characterization of a coactivator for the steroid hormone receptor superfamily. Science 270: 1354-1357, 1995. 2. Onate SA, Boonyaratanakornkit V, Spencer TE, Tsai SY, Tsai MJ, Edwards DP et al. (1998). "The steroid receptor coactivator-1 contains multiple receptor interacting and activation domains that cooperatively enhance the activation function 1 (AF1) and AF2 domains of steroid receptors". J Biol Chem 273 (20): 12101-8.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: NCOA1

Background:

Synonyms: Nuclear receptor coactivator 1,NCoA-1,2.3.1.48,Class E basic helix-loop-helix protein 74,bHLHe74,Protein Hin-2,RIP160,Renal carcinoma antigen NY-REN-52,Steroid receptor coactivator 1,SRC-1,NCOA1,BHLHE74, SRC1,

Tissue Specificity: Widely expressed. .

Background: The nuclear receptor coactivator 1 (NCOA1), also known as SRC1, is a transcriptional coregulatory protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCOA1 is recruited to DNA promotion sites by ligand-activated nuclear receptors. NCOA1, in turn, acylates histones, which makes downsteam DNA more accessible to transcription. Hence, NCOA1 assists nuclear receptors in the upregulation of DNA expression. It has been found that NCOA1 can enhance the transcriptional activity of ligand-bound PGR but does not alter the basal activity of the target promoter. It also enhances estrogen receptor, glucocorticoid receptor, thyroid hormone receptor, and retinoid X receptor transcriptional activities through their cognate DNA response elements in the presence of hormone. What's more, SRC1 may play a role as a bridging molecule between nuclear hormone receptors and general transcription factors.

Sequence Similarities: Belongs to the SRC/p160 nuclear receptor coactivator family.

Molecular Weight: 157 kDa

Gene ID: 8648

UniProt: Q15788

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha