FRZB antibody (AA 176-325)

Catalog No. ABIN3042406

Product Details anti-FRZB Antibody

Target: FRZB (Frizzled-Related Protein (FRZB))

Binding Specificity: AA 176-325

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRZB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 3(FRZB) detection. Tested with WB, ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Secreted frizzled-related protein 3(FRZB) detection. Tested with WB, ELISA in Human.
Gene Name: frizzled-related protein
Protein Name: Secreted frizzled-related protein 3

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for FRZB is approximately 0.1 ng/lane under reducing conditions.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: FRZB (FRZB Products)

Synonyms: Frp antibody, Sfrp3 antibody, frezzled antibody, fritz antibody, frzb-1 antibody, FRE antibody, FRITZ antibody, FRP-3 antibody, FRZB-1 antibody, FRZB-PEN antibody, FRZB1 antibody, FZRB antibody, OS1 antibody, SFRP3 antibody, SRFP3 antibody, hFIZ antibody, Frzb-1 antibody, frizzled-related protein antibody, Frzb antibody, FRZB antibody

Background: FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Synonyms: FIZ antibody|FRE antibody|Frezzled antibody|Fritz antibody|Frizzled-related protein 1 antibody|FRP antibody|FrzB 1 antibody|FRZB antibody|FRZB1 antibody|OTTMUSP00000014350 antibody|RP23-33L19.1 antibody|Secreted frizzled related protein 3 [Precursor] antibody|Secreted frizzled related sequence protein 3 antibody|sFRP 3 antibody|SFRP antibody

Gene ID: 2487

UniProt: Q92765

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation