HEXB antibody (AA 381-556)

Catalog No. ABIN3042442

This Rabbit Polyclonal antibody specifically detects HEXB in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for HEXB antibody (AA 381-556) (ABIN3042442)

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEXB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HEXB Antibody

Binding Specificity: AA 381-556

Purpose: Anti-HEXB Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human HEXB shares 75%,73% amino acid (aa) sequences identity with mouse,rat HEXB,respectively.

Characteristics: Anti-HEXB Antibody. Tested in IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, HumanWestern blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HEXB

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Alternative Name: HEXB

Background:

Background: Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity.

Gene Full Name: hexosaminidase subunit beta

Sequence Similarities: Belongs to the glycosyl hydrolase 20 family.

Molecular Weight: 63 kDa

Gene ID: 3074

UniProt: P07686

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process