HSD17B10 antibody (AA 48-261)

Catalog No. ABIN3042460

This Rabbit Polyclonal antibody specifically detects HSD17B10 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for HSD17B10 antibody (AA 48-261) (ABIN3042460)

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-HSD17B10 Antibody

Binding Specificity: AA 48-261

Purpose: Anti-ERAB/HSD17B10 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ERAB/HSD17B10 Antibody Picoband® (ABIN3042460). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Deutschmann AJ, et al. Mutation or knock-down of 17β-hydroxysteroid dehydrogenase type 10 cause loss of MRPP1 and impaired processing of mitochondrial heavy strand transcripts. Hum Mol Genet, 2014 Jul 1. 2. Yang SY, et al. Roles of 17β-hydroxysteroid dehydrogenase type 10 in neurodegenerative disorders. J Steroid Biochem Mol Biol, 2014 Sep.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HSD17B10

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Alternative Name: HSD17B10

Background:

Synonyms: 3-hydroxyacyl-CoA dehydrogenase type-2,1.1.1.35,17-beta-hydroxysteroid dehydrogenase 10,17-beta-HSD 10,1.1.1.51,3-hydroxy-2-methylbutyryl-CoA dehydrogenase,1.1.1.178,3-hydroxyacyl-CoA dehydrogenase type II,Endoplasmic reticulum-associated amyloid beta-peptide-binding protein,Mitochondrial ribonuclease P protein 2,Mitochondrial RNase P protein 2,Short chain dehydrogenase/reductase family 5C member 1,Short-chain type dehydrogenase/reductase XH98G2,Type II HADH,HSD17B10,ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2,

Tissue Specificity: Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. .

Background: ERAB is also known as HSD17B10 or HADH2. This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Molecular Weight: 25 kDa

Gene ID: 3028

UniProt: Q99714