HSP27 antibody (AA 1-205)

Catalog No. ABIN3042464

Product Details anti-HSP27 Antibody

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Binding Specificity: AA 1-205

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: heat shock 27 kDa protein 1
Protein Name: Heat shock protein beta-1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Hsp27 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 (HSPB1 Products)

Synonyms: CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

Background: HSPB1(Heat shock 27 kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Synonyms: Heat shock 27 kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|Heat Shock Protein27 antibody|Heat Shock Protein27 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|Hsp 25 antibody|Hsp 27 antibody|Hsp 27 antibody|Hsp 28 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp B1 antibody|Hsp25 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|Hsp28 antibody|HspB1 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling