KIN antibody (AA 3-202)

Catalog No. ABIN3042474

Product Details anti-KIN Antibody

Target: KIN (KIN, Antigenic Determinant of RecA Protein Homolog (KIN))

Binding Specificity: AA 3-202

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for DNA/RNA-binding protein KIN17(KIN) detection. Tested with WB, IHC-P in Human,Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for DNA/RNA-binding protein KIN17(KIN) detection. Tested with WB, IHC-P in Human,Mouse.
Gene Name: KIN, antigenic determinant of recA protein homolog (mouse)
Protein Name: DNA/RNA-binding protein KIN17

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KIN recombinant protein (Position: K3-K202). Human KIN shares 98% amino acid (aa) sequence identity with mouse KIN.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for KIN

Target: KIN (KIN, Antigenic Determinant of RecA Protein Homolog (KIN))

Alternative Name: KIN (KIN Products)

Synonyms: Kin17 antibody, BTCD antibody, KIN17 antibody, Kin17 DNA and RNA binding protein antibody, Kin17 DNA and RNA binding protein S homeolog antibody, Kin antibody, kin.S antibody, KIN antibody

Background: DNA/RNA-binding protein KIN17, also known as BTCD or KIN17, is a protein that in humans is encoded by the KIN gene. This gene is mapped to 10p14. The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants.

Synonyms: antigenic determinant of recA protein homolog antibody|Binding to curved DNA antibody|BTCD antibody|DNA/RNA-binding protein KIN17 antibody|HsKin17 protein antibody|KIN antibody|KIN antigenic determinant of recA protein homolog mouse antibody|KIN17 antibody|Kin17 protein antibody|KIN17_HUMAN antibody

Gene ID: 22944

UniProt: O60870