SLUG antibody (Middle Region)

Catalog No. ABIN3042486

Cited in 1 publication. The Rabbit Polyclonal anti-SLUG antibody (ABIN3042486) specifically detects SLUG in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SLUG antibody (Middle Region) (ABIN3042486)

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLUG Antibody

Binding Specificity: Middle Region

Purpose: Anti-SLUG/SNAI2 Antibody

Sequence: KLSDPHAIEA EKFQCNLCNK TYSTFSGLAK HKQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-SLUG/SNAI2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SLUG, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SLUG antibody (ABIN3042486)

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." in: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

Target Details for SLUG

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Alternative Name: SNAI2

Background:

Background: SLUG is also known as SNAI2. This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Gene Full Name: snail family transcriptional repressor 2

Sequence Similarities: Belongs to the snail C2H2-type zinc-finger protein family.

Molecular Weight: 30 kDa

Gene ID: 6591

UniProt: O43623