CD43 antibody (AA 272-400)

Catalog No. ABIN3042491

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Binding Specificity: AA 272-400

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: sialophorin
Protein Name: Leukosialin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD43 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: SPN (SPN Products)

Synonyms: CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody

Background: CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Synonyms: CD 43 antibody|CD43 antibody|CD43 antigen antibody|Galactoglycoprotein antibody|Galactoglycoprotein antibody|GALGP antibody|GPL 115 antibody|GPL115 antibody|Human gene for sialophorin antibody|Leucocyte sialoglycoprotein antibody|Leucocyte sialoglycoprotein antibody|LEUK_HUMAN antibody|Leukocyte sialoglycoprotein antibody|Leukosialin antibody|LSN antibody|Sialophorin (leukosialin) antibody|Sialophorin antibody|SPN antibody

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity