RAB8A antibody (C-Term)

Catalog No. ABIN3042554

The Rabbit Polyclonal anti-RAB8A antibody has been validated for WB and IHC. It is suitable to detect RAB8A in samples from Human.

Quick Overview for RAB8A antibody (C-Term) (ABIN3042554)

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB8A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAB8A Antibody

Binding Specificity: AA 170-183, C-Term

Purpose: Anti-Ras-related protein Rab-8A RAB8A Antibody Picoband®

Sequence: KAKMDKKLEG NSPQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Ras-related protein Rab-8A RAB8A Antibody (ABIN3042554). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB8A, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. "Entrez Gene: RAB8A RAB8A, member RAS oncogene family" 2. Tsang, W. Y., Bossard, C., Khanna, H., Peranen, J., Swaroop, A., Malhotra, V., Dynlacht, B. D. CP110 suppresses primary cilia formation through its interaction with CEP290, a protein deficiency in human ciliary disease. Dev. Cell 15: 187-197, 2008. 3. Nimmo ER, Sanders PG, Padua RA, Hughes D, Williamson R, Johnson KJ (October 1991). "The MEL gene: a new member of the RAB/YPT class of RAS-related genes". Oncogene 6 (8): 1347-51.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB8A

Target: RAB8A (RAB8A, Member RAS Oncogene Family (RAB8A))

Alternative Name: RAB8A

Background:

Synonyms: Ras-related protein Rab-8A,Oncogene c-mel,RAB8A,MEL, RAB8,

Tissue Specificity: Expressed in brain, placenta and lung. .

Background: Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. It is mapped to 19p13.12. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. RAB8A regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of RAB8A into the cilium during assembly of this organelle. RAB8A has been shown to interact with Optineurin and MAP4K2.

Sequence Similarities: Belongs to the beta-catenin family.

Molecular Weight: 24 kDa

UniProt: P61006

Pathways: M Phase, Regulation of long-term Neuronal Synaptic Plasticity, SARS-CoV-2 Protein Interactome