ATG5 antibody (Middle Region)

Catalog No. ABIN3042571

This Rabbit Polyclonal antibody specifically detects ATG5 in WB, IHC, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 6+ publications

Quick Overview for ATG5 antibody (Middle Region) (ABIN3042571)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ATG5 Antibody

Binding Specificity: Middle Region

Purpose: Anti-APG5L/ATG5 Antibody

Sequence: DRFDQFWAIN RKLMEY

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse sequence,and different from the related rat sequence by one amino acid.

Characteristics: Anti-APG5L/ATG5 Antibody. Tested in Flow Cytometry, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human APG5L, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-ATG5 antibody (ABIN3042571)

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Shao, Chen, Chen, Zhang, Lin, Cai, Yin, Tao, Liao, Zhao, Mai, He, He, Cui: "Association between genetic polymorphisms in the autophagy-related 5 gene promoter and the risk of sepsis." in: Scientific reports, Vol. 7, Issue 1, pp. 9399, (2017) (PubMed).

Sun, Gao, Meng, Xu, Wang, Gu, Guo, Shao, Yan, Jiang, Zheng: "Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy." in: Frontiers in pharmacology, Vol. 8, pp. 524, (2017) (PubMed).

Yang, Sheng, Yang, Li, Qin, Yu, Zhang: "Endocan silencing induces programmed cell death in hepatocarcinoma." in: Oncology letters, Vol. 14, Issue 5, pp. 5333-5339, (2017) (PubMed).

Song, Li, Lin, Cao: "Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 7615-22, (2015) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background:

Background: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Gene Full Name: autophagy related 5

Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family.

Molecular Weight: 50-52 kDa

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy