Nicastrin antibody (N-Term)

Catalog No. ABIN3042580

This Rabbit Polyclonal antibody specifically detects Nicastrin in WB. It exhibits reactivity toward Human.

Quick Overview for Nicastrin antibody (N-Term) (ABIN3042580)

Target: Nicastrin (NCSTN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicastrin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nicastrin Antibody

Binding Specificity: AA 72-88, N-Term

Purpose: Anti-Nicastrin/NCSTN Antibody Picoband®

Sequence: VIHVVEKEED LQWVLTD

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Nicastrin/NCSTN Antibody (ABIN3042580). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nicastrin, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: NCSTN nicastrin" 2. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (October 1996)."Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res. 3 (5): 321-9, 341-54. 3. Zhang YW, Luo WJ, Wang H, Lin P, Vetrivel KS, Liao F, Li F, Wong PC, Farquhar MG, Thinakaran G, Xu H (April 2005)."Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components".J. Biol. Chem. 280 (17): 17020-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Nicastrin

Target: Nicastrin (NCSTN)

Alternative Name: NCSTN

Background:

Synonyms: Nicastrin,NCSTN,KIAA0253,UNQ1874/PRO4317,

Tissue Specificity: Widely expressed. .

Background: Nicastrin, also known as?NCSTN, is a?protein?that is a part of the gamma secretase?protein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretase?protein complex, all of which undergo significant post-translational modification?before becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex.?The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly.?The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, an?enzyme?involved in the degradation of amyloid beta fragment.

Sequence Similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

Molecular Weight: 120-130 kDa

UniProt: Q92542

Pathways: Notch Signaling, Neurotrophin Signaling Pathway