CDK5 antibody (C-Term)

Catalog No. ABIN3042679

This Rabbit Polyclonal antibody specifically detects CDK5 in WB. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for CDK5 antibody (C-Term) (ABIN3042679)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDK5 Antibody

Binding Specificity: AA 273-292, C-Term

Purpose: Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody Picoband®

Sequence: QRISAEEALQ HPYFSDFCPP

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody (ABIN3042679). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk5, identical to the related rat and mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Demetrick, D. J., Zhang, H., Beach, D. H.Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes.Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Ohshima, T., Nagle, J. W., Pant, H. C., Joshi, J. B., Kozak, C. A., Brady, R. O., Kulkarni, A. B.Molecular cloning and chromosomal mapping of the mouse cyclin-dependent kinase 5 gene.Genomics 28: 585-588, 1995. 3. Bibb, J. A., Snyder, G. L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A. A., Tsai, L.-H., Kwon, Y. T., Girault, J.-A., Czernik, A. J., Huganir, R. L., Hemmings, H. C., Jr., Nairn, A. C., Greengard, P.Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons.Nature 402: 669-671, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CDK5 antibody (ABIN3042679)

Li, Zhang, Zhu, Zhang, Lin, Xu, Yang, Hao, Zhang: "A new treatment for cognitive disorders related to in utero exposure to alcohol." in: Neural regeneration research, Vol. 8, Issue 18, pp. 1702-13, (2014) (PubMed).

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background:

Synonyms: Cyclin-dependent-like kinase 5,2.7.11.1,Cell division protein kinase 5,Serine/threonine-protein kinase PSSALRE,Tau protein kinase II catalytic subunit,TPKII catalytic subunit,CDK5,CDKN5,

Tissue Specificity: Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary. .

Background: CDK5, Cyclin-Dependent Kinase5, is also know as PSSALRE. The p34 (CDC2) protein kinase regulates important transitions in the eukaryotic cell cycle. The PSSALRE expression is in all human tissues and cell lines tested. The the CDK5 gene is mapped to chromosome 7q36. Cyclin-dependent kinase-5 is predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau. CDK5 is required for proper development of the mammalian central nervous system. To be activated, CDK5 must associate with its regulatory subunit, p35 (CDK5R1). CDK5 can phosphorylate DARPP32 at threonine-75, converting it into an inhibitor of PKA.

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Molecular Weight: 34 kDa

UniProt: Q00535

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis