IGFBP5 antibody (N-Term)

Catalog No. ABIN3042733

This Rabbit Polyclonal antibody specifically detects IGFBP5 in WB and ELISA. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for IGFBP5 antibody (N-Term) (ABIN3042733)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IGFBP5 Antibody

Binding Specificity: N-Term

Purpose: Anti-IGFBP5 Antibody

Sequence: QGLRCLPRQD EEKPLHALLH GRGVCLNEKS YREQVKIER

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by two amino acids.

Characteristics: Anti-IGFBP5 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human IGFBP5, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: ELISA, 0.1-0.5 μg/mL, -Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IGFBP5 antibody (ABIN3042733)

Wan, Ma, Mei, Shan: "The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 150, (2010) (PubMed).

Hou, Zhang, Liu, Meng, Qiao: "Expressions of IGFBP-5, cFLIP in cervical intraepithelial neoplasia, cervical carcinoma and their clinical significances: a molecular pathology." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 70, (2009) (PubMed).

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP5

Background:

Background: Insulin-like growth factor-binding protein 5?is a?protein?that in humans is encoded by the?IGFBP5?gene. The expression of?IGFBP5?by stable transfection and adenovirus-mediated infection is inhibitory to growth in 2 human breast cancer cell lines.?IGFBP5?expression leads to G2/M cell cycle arrest and apoptosis. Stable expression of?IGFBP5?in the breast cancer cell lines also inhibits the formation and growth of tumors following injection in athymic mice. It is concluded that IGFBP5?is a growth inhibitor and proapoptotic agent in breast cancer cells. Additionally, IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. It has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21? kDa fragment (1,?2).

Gene Full Name: insulin like growth factor binding protein 5

Molecular Weight: 23 kDa

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding