CIB1 antibody (AA 6-191)

Catalog No. ABIN3042748

This Rabbit Polyclonal antibody specifically detects CIB1 in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CIB1 antibody (AA 6-191) (ABIN3042748)

Target: CIB1 (Calcium and Integrin-Binding Protein 1 (CIB1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CIB1 Antibody

Binding Specificity: AA 6-191

Purpose: Anti-CIB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CIB1 Antibody Picoband® (ABIN3042748). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CIB1 recombinant protein (Position: S6-L191). Human CIB1 shares 93.5% amino acid (aa) sequence identity with both mouse and rat CIB1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: CIB1 calcium and integrin binding 1 (calmyrin)". 2. Hattori A, Seki N, Hayashi A, Kozuma S, Saito T (Aug 2000). "Genomic structure of mouse and human genes for DNA-PKcs interacting protein (KIP)". DNA Seq 10 (6): 415-8. 3. Naik UP, Patel PM, Parise LV (Apr 1997). "Identification of a novel calcium-binding protein that interacts with the integrin alphaIIb cytoplasmic domain". J Biol Chem 272 (8): 4651-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CIB1

Target: CIB1 (Calcium and Integrin-Binding Protein 1 (CIB1))

Alternative Name: CIB1

Background:

Synonyms: Calcium and integrin-binding protein 1,CIB,Calcium- and integrin-binding protein,CIBP,Calmyrin,DNA-PKcs-interacting protein,Kinase-interacting protein,KIP,SNK-interacting protein 2-28,SIP2-28,CIB1,CIB, KIP, PRKDCIP,

Tissue Specificity: Detected in platelets and in cell lines of megakaryocytic and erythrocytic lineages. Both isoform 1 and isoform 2 are detected in various cancer cell lines, with isoform 2 being the predominant form (at protein level). Ubiquitously expressed. .

Background: Calcium and integrin-binding protein 1 is a protein that in humans is encoded by the CIB1 gene. This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. And this protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. Moreover, the encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Belongs to the MPI phosphatase family.

Molecular Weight: 27 kDa

Gene ID: 10519

UniProt: Q99828