TNF alpha antibody (AA 80-235)

Catalog No. ABIN3042817

This Rabbit Polyclonal antibody specifically detects TNF alpha in WB, ELISA and IHC. It exhibits reactivity toward Mouse and has been mentioned in 51+ publications.

Quick Overview for TNF alpha antibody (AA 80-235) (ABIN3042817)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 80-235

Purpose: Anti-TNF alpha Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-< a href="https://www.bosterbio.com/anti-tnf-alpha-picoband-trade-antibody-a00002-2-boster.html" style="color:#EA8D28">TNF alpha Antibody (ABIN3042817). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Mouse
1. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984. 2. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide. Carrier free (No BSA)

Preservative: Sodium azide, Without preservative

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-TNF alpha antibody (ABIN3042817)

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Sun, Xue, Xue, Ren, Wu, Wang: "Acetylpuerarin protects against OGD-induced cell injury in BV2 microglia by inhibiting HMGB1 release." in: Die Pharmazie, Vol. 73, Issue 2, pp. 92-97, (2018) (PubMed).

Gu, Ding, Tang, Lei, Jiang, Xu: "A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis." in: Frontiers in pharmacology, Vol. 9, pp. 331, (2018) (PubMed).

Wang, Li, Chen, Jiang, Lu, Zhao: "Hydrogen sulfide accelerates wound healing in diabetic rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5097-104, (2016) (PubMed).

Pan, Wang, Pu, Yao, Wang: "Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 2134-40, (2016) (PubMed).

Chen, Wang, Li, Jiang, Lu, Zhou: "Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9726503, (2016) (PubMed).

Ke, Li, Chen: "Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1563-1572, (2016) (PubMed).

Liu, Lv, Ning, Yang, Zhu: "Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model." in: Experimental and therapeutic medicine, Vol. 11, Issue 6, pp. 2284-2292, (2016) (PubMed).

Li, Tan, Tong, Han, Zhang, Liu, Sun: "Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A." in: American journal of translational research, Vol. 8, Issue 5, pp. 2210-21, (2016) (PubMed).

Zhang, Liu, Zhou, Hu, Qian: "Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation." in: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3181-3188, (2016) (PubMed).

Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

Kan, Zhou, Jin, Yang: "Effects of PDTC on NF-?B expression and apoptosis in rats with severe acute pancreatitis-associated lung injury." in: International journal of clinical and experimental medicine, Vol. 8, Issue 3, pp. 3258-70, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." in: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Li, Qiu, Wang, Niu, Pan: "Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury." in: Food & function, Vol. 6, Issue 2, pp. 470-8, (2015) (PubMed).

Xia, Li, Xiang, Chang, Xia, Han: "Neuroprotective effects of an aqueous extract of Futokadsura stem in an A?-induced Alzheimer's disease-like rat model." in: The Chinese journal of physiology, Vol. 58, Issue 2, pp. 104-13, (2015) (PubMed).

Xie, Zhang, Li, Xu: "Inhibition of inflammatory cytokines after early decompression may mediate recovery of neurological function in rats with spinal cord injury." in: Neural regeneration research, Vol. 10, Issue 2, pp. 219-24, (2015) (PubMed).

Wu, Su, Li, Wu, Wu, Tang: "Cantharidin-induced liver injuries in mice and the protective effect of vitamin C supplementation." in: International immunopharmacology, Vol. 28, Issue 1, pp. 182-7, (2015) (PubMed).

Cheng, Xiao, Ainiwaer, Wang, Wu, Mao, Yang, Bao: "Molecular responses of radiation-induced liver damage in rats." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2592-600, (2015) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF

Background:

Synonyms: Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Tumor necrosis factor, membrane form,N-terminal fragment,NTF,Intracellular domain 1,ICD1,Intracellular domain 2,ICD2,C-domain 1,C-domain 2,Tumor necrosis factor, soluble form,Tnf,Tnfa, Tnfsf2,

Tissue Specificity: Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).

Background: TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Molecular Weight: 17 kDa

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome