COMT antibody (N-Term)

Catalog No. ABIN3042837

Product Details anti-COMT Antibody

Target: COMT (Catechol-O-Methyltransferase (COMT))

Binding Specificity: AA 94-108, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Catechol O-methyltransferase(COMT) detection. Tested with WB, IHC-P in Human.

Sequence: DKKGKIVDAV IQEHQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Catechol O-methyltransferase(COMT) detection. Tested with WB, IHC-P in Human.
Gene Name: catechol-O-methyltransferase
Protein Name: Catechol O-methyltransferase

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human COMT(94-108aa DKKGKIVDAVIQEHQ).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: COMT (COMT Products)

Synonyms: Comt1 antibody, D16Wsu103e antibody, D330014B15Rik antibody, COMT antibody, mb-comt antibody, s-comt antibody, comt antibody, MGC154444 antibody, zgc:114157 antibody, si:dkey-13a21.15 antibody, zK13A21.15 antibody, zgc:162236 antibody, catechol-O-methyltransferase antibody, microRNA 4761 antibody, catechol-O-methyltransferase S homeolog antibody, methyltransferase antibody, catechol-o-methyltransferase antibody, catechol-O-methyltransferase a antibody, catechol-O-methyltransferase b antibody, catechol O-methyltransferase antibody, COMT antibody, Comt antibody, MIR4761 antibody, comt antibody, comt.2 antibody, comt.S antibody, Rv1703c antibody, Mb1729c antibody, comta antibody, comtb antibody, LOC100354142 antibody

Background: Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form(S-COMT) and a membrane-bound form(MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.

Synonyms: Catechol O methyltransferase antibody|Catechol O-methyltransferase antibody|COMT antibody|COMT_HUMAN antibody|EC 2.1.1.6 antibody

UniProt: P21964

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome