Factor VII antibody (C-Term)

Catalog No. ABIN3042925

This Rabbit Polyclonal antibody specifically detects Factor VII in WB. It exhibits reactivity toward Human.

Quick Overview for Factor VII antibody (C-Term) (ABIN3042925)

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VII antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Factor VII Antibody

Binding Specificity: AA 441-453, C-Term

Purpose: Anti-Factor VII/F7 Antibody Picoband®

Sequence: SQYIEWLQKL MRS

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Rabbit IgG polyclonal antibody for Coagulation factor VII(F7) detection. Tested with WB in Human.
Gene Name: coagulation factor VII(serum prothrombin conversion accelerator)
Protein Name: Coagulation factor VII

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Di Bitondo, R., Hall, A. J., Peake, I. R., Iacoviello, L., Winship, P. R. Oestrogenic repression of human coagulation factor VII expression mediated through an oestrogen response element sequence motif in the promoter region. Hum. Molec. Genet. 11: 723-731, 2002. 2. Millar, D. S., Kemball-Cook, G., McVey, J. H., Tuddenham, E. G. D., Mumford, A. D., Attock, G. B., Reverter, J. C., Lanir, N., Parapia, L. A., Reynaud, J., Meili, E., von Felton, A., Martinowitz, U., Prangnell, D. R., Krawczak, M., Cooper, D. N. Molecular analysis of the genotype-phenotype relationship in factor VII deficiency. Hum. Genet. 107: 327-342, 2000. 3. Zur, M., Nemerson, Y. Tissue factor pathways of blood coagulation.In: Bloom, A. L., Thomas, D. P. : Haemostasis and thrombosis. Edinburgh: Churchill Livingstone (pub.) 1981.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Alternative Name: F7

Background:

Synonyms: Coagulation factor VII,3.4.21.21,Proconvertin,Serum prothrombin conversion accelerator,SPCA,Eptacog alfa,Factor VII light chain,Factor VII heavy chain,F7,

Tissue Specificity: Plasma.

Background: F7 (Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34 (Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown (Zur and Nemerson, 1981). Di Bitondo et al. (2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.

Sequence Similarities: Belongs to the peptidase S1 family.

Molecular Weight: 52 kDa

UniProt: P08709

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling