CRTC2 antibody (C-Term)

Catalog No. ABIN3042964

This Rabbit Polyclonal antibody specifically detects CRTC2 in WB. It exhibits reactivity toward Human.

Quick Overview for CRTC2 antibody (C-Term) (ABIN3042964)

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRTC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CRTC2 Antibody

Binding Specificity: AA 595-611, C-Term

Purpose: Anti-TORC2/CRTC2 Antibody Picoband®

Sequence: QDPHTFNHQN LTHCSRH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TORC2/CRTC2 Antibody (ABIN3042964). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TORC2, different from the related rat sequence by three amino acids, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Iourgenko, V., Zhang, W., Mickanin, C., Daly, I., Jiang, C., Hexham, J. M., Orth, A. P., Miraglia, L., Meltzer, J., Garza, D., Chirn, G.-W., McWhinnie, E., and 9 others. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells. Proc. Nat. Acad. Sci. 100: 12147-12152, 2003. 2. Liu, Y., Dentin, R., Chen, D., Hedrick, S., Ravnskjaer, K., Schenk, S., Milne, J., Meyers, D. J., Cole, P., Yates, J., III, Olefsky, J., Guarente, L., Montminy, M. A fasting inducible switch modulates gluconeogenesis via activator/coactivator exchange. Nature 456: 269-273, 2008. 3. Wang, Y., Vera, L., Fischer, W. H., Montminy, M. The CREB coactivator CRTC2 links hepatic ER stress and fasting gluconeogenesis. Nature 460: 534-537, 2009.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Alternative Name: CRTC2

Background:

Synonyms: CREB-regulated transcription coactivator 2,Transducer of regulated cAMP response element-binding protein 2,TORC-2,Transducer of CREB protein 2,CRTC2,TORC2,

Tissue Specificity: Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. .

Background: CREB regulated transcription coactivator 2, also known as CRTC2, is a protein which in humans is encoded by the CRTC2 gene. This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5.

Sequence Similarities: Belongs to the TORC family.

Molecular Weight: 80 kDa

Pathways: AMPK Signaling, Carbohydrate Homeostasis