SPTLC1 antibody (Middle Region)

Catalog No. ABIN3043056

The Rabbit Polyclonal anti-SPTLC1 antibody (ABIN3043056) specifically detects SPTLC1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SPTLC1 antibody (Middle Region) (ABIN3043056)

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPTLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SPTLC1 Antibody

Binding Specificity: AA 138-154, Middle Region

Purpose: Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody

Sequence: FYGTFDVHLD LEDRLAK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Serine palmitoyltransferase 1 SPTLC1 Antibody (ABIN3043056). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SPTLC1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Alternative Name: SPTLC1

Background:

Synonyms: Serine palmitoyltransferase 1,2.3.1.50,Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT 1,SPT1,SPTLC1,LCB1,

Tissue Specificity: Widely expressed. Not detected in small intestine. .

Background: SPTLC1 (Serine palmitoyltransferase, long chain base subunit 1), also known as SPT1, LCB1, is a protein which in humans is encoded by the SPTLC1 gene. Dawkins et al. (2001)noted that the SPTLC1 gene maps to chromosome 9q22.1-q22.3. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

Sequence Similarities: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Molecular Weight: 56 kDa

UniProt: O15269