CD14 antibody (Middle Region)

Catalog No. ABIN3043216

This Rabbit Polyclonal antibody specifically detects CD14 in WB. It exhibits reactivity toward Mouse and has been mentioned in 6+ publications.

Quick Overview for CD14 antibody (Middle Region) (ABIN3043216)

Target: CD14

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD14 Antibody

Binding Specificity: AA 152-165, Middle Region

Purpose: Anti-CD14 Antibody Picoband®

Sequence: TRDAWLAELQ QWLK

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD14 Antibody (ABIN3043216). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD14.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. Goyert, S. M., Ferrero, E., Rettig, W. J., Yenamandra, A. K., Obata, F., Le Beau, M. M. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors. Science 239: 497-500, 1988. 2. LeVan, T. D., Bloom, J. W., Bailey, T. J., Karp, C. L., Halonen, M., Martinez, F. D., Vercelli, D. A common single nucleotide polymorphism in the CD14 promoter decreases the affinity of Sp protein binding and enhances transcriptional activity. J. Immun. 167: 5838-5844, 2001. 3. Wright, S. D. CD14: a leukocyte membrane protein that functions in the response to endotoxin. (Abstract) FASEB J. 4: A1848 only, 1990.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CD14 antibody (ABIN3043216)

Wu, Yang, Qi, Zhou, Hu, Wang: "ANTI-INFLAMMATORY ACTIVITY OF PLATYCODIN D ON ALCOHOL-INDUCED FATTY LIVER RATS VIA TLR4-MYD88-NF-κB SIGNAL PATH." in: African journal of traditional, complementary, and alternative medicines : AJTCAM, Vol. 13, Issue 4, pp. 176-183, (2017) (PubMed).

Liu, Wang, Ye, Yin, Chen: "Distinct molecular basis for endothelial differentiation: gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells." in: Cellular immunology, Vol. 289, Issue 1-2, pp. 7-14, (2014) (PubMed).

Wu, Liu, Zhang, Xing, Zheng, Ji: "Chinese medicinal formula, Qinggan Huoxue Recipe protects rats from alcoholic liver disease via the lipopolysaccharide-Kupffer cell signal conduction pathway." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 363-370, (2014) (PubMed).

Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

Wang, Xiao, Huang, Gong, Li: "Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats." in: World journal of gastroenterology, Vol. 19, Issue 15, pp. 2319-30, (2013) (PubMed).

Zhao, Zhao, Feng, Zhang, Zhuang, Zhao, Li, Li, Liu: "Detection of vulnerable atherosclerotic plaque and prediction of thrombosis events in a rabbit model using 18F-FDG -PET/CT." in: PLoS ONE, Vol. 8, Issue 4, pp. e61140, (2013) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Monocyte differentiation antigen CD14,Myeloid cell-specific leucine-rich glycoprotein,CD14,Cd14,

Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Background: CD14, Cluster of differentiation 14, single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Molecular Weight: 50 kDa

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades