IL-3 antibody (AA 28-166)

Catalog No. ABIN3043247

Product Details anti-IL-3 Antibody

Target: IL-3 (Interleukin 3 (IL-3))

Binding Specificity: AA 28-166

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, ELISA in Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, ELISA in Rat.
Gene Name: interleukin 3
Protein Name: Interleukin-3

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for IL-3 is approximately 0.25 ng/lane under reducing conditions.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3 (IL-3 Products)

Synonyms: BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody

Background: Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Synonyms: Colony stimulating factor multiple antibody|Hematopoietic growth factor antibody|IL 3 antibody|IL-3 antibody|IL3 antibody|IL3_HUMAN antibody|Interleukin 3 (colony stimulating factor, multiple) antibody|Interleukin 3 antibody|Interleukin-3 antibody|Mast cell growth factor antibody|MCGF antibody|MGC79398 antibody|MGC79399 antibody|Multi CSF antibody|Multilineage colony stimulating factor antibody|Multipotential colony stimulating factor antibody|Multipotential colony-stimulating factor antibody|OTTHUMP00000065963 antibody|P cell stimulating factor antibody|P-cell-stimulating factor antibody

Gene ID: 24495

UniProt: P04823

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy