ITGA5 antibody (AA 787-992)

Catalog No. ABIN3043256

There are 3+ publications for this product available. The Rabbit Polyclonal anti-ITGA5 antibody is suitable to detect ITGA5 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for ITGA5 antibody (AA 787-992) (ABIN3043256)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ITGA5 Antibody

Binding Specificity: AA 787-992

Purpose: Anti-Integrin alpha 5/ITGA5 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Integrin alpha 5 shares 86% amino acid (aa) sequence identity with mouse Integrin alpha 5.

Characteristics: Anti-Integrin alpha 5/ITGA5 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Integrin alpha 5 recombinant protein (Position: E787-E992). Human Integrin alpha 5 shares 86% amino acid (aa) sequence identity with mouse Integrin alpha 5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-ITGA5 antibody (ABIN3043256)

Chen, Sun, Liu, Tong, Meng: "Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144845, (2015) (PubMed).

Zhang, Ren, Luo, Fan, Liu, Liang, Ma, Yu, Bai: "A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92252, (2014) (PubMed).

Huang, Lian, Chen, Yang, Yang: "Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system." in: Acta histochemica et cytochemica, Vol. 42, Issue 5, pp. 129-36, (2009) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGA5

Background:

Background: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Gene Full Name: integrin subunit alpha 5

Sequence Similarities: Belongs to the integrin alpha chain family.

Molecular Weight: 130 kDa

Gene ID: 3678

UniProt: P08648

Pathways: Integrin Complex